Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia
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چکیده
منابع مشابه
Influence of convection in the growth medium on oxygen tensions in multicellular tumor spheroids.
Recessed microelectrodes have been used to measure ox ygen tensions (PO2) in tumor spheroids of EMT6/Ro cells which were grown in spinner flasks. For the PO2 measure ments, the spheroids were put into a thermostatically controlled measuring chamber that was designed to create conditions similar to those to which the spheroids are exposed in spinner flasks. Under these conditions, different elec...
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The in vitro growth of small (0.05 to 3 mm diameter) avascular multicellular tumor spheroids from six rodent and two human tumor lines has been analyzed. Surprisingly, the radial increase of multicellular tumor spheroids is linear with time after a brief initial period of geometric growth. These multicellular tumor spheroids are shown to have a constant thickness of proliferative outer crust an...
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Most organisms grow according to simple laws, which in principle can be derived from energy conservation and scaling arguments, critically dependent on the relation between the metabolic rate B of energy flow and the organism mass m. Although this relation is generally recognized to be of the form B(m) = m, the specific value of the exponent p is the object of an ongoing debate, with many mecha...
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Many chemotherapeutic strategies have failed due to pronounced diffusion limitations in tumors induced by poor vasculaturization. Diffusion limitations, present in threedimensional tissue, produce nutrient deficient regions that eventually become necrotic in tumors in vivo. Multicellular spheroids are better models of in vivo tumors than monolayer cultures because they mimic the heterogeneous m...
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Background Advantages of microencapsulation as a 3D growth system are chemically and spatially defined 3D network of extracellular matrix components, cell-to-cell and cell-tomatrix interactions governing differentiation, proliferation and cell function in vivo. The study is aimed at i) optimization of techniques for preparing microcapsules; ii) generation of multicellular tumor spheroids (MTS) ...
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ژورنال
عنوان ژورنال: Oncology Letters
سال: 2017
ISSN: 1792-1074,1792-1082
DOI: 10.3892/ol.2017.7465